Gastrointestinal smooth muscle spasm is the major cause of symptoms of different gastrointestinal disorders. This study was undertaken to examine the effects of Jamun fruit (Eugenia jambolana) extract. The experiment was performed on forty rabbits; properly dissected. Study was performed on Power Lab System. Acetylcholine, histamine, serotonin, and calcium were used in increasing doses to obtain significant contractions in the ilium until the ceiling effect was observed. Thereafter, these drugs were used in the presence of different doses of Jamun extract. Shift of different concentration-response curves in each group was obtained. Percentage inhibition was determined in each group.