Background:
The present study focuses on the anesthetic and antibacterial effect of ketamine which is a commonly used general anesthetic.
Methods:
Five derivatives of ketamine i.e. Rxn-102, Rxn-103, Rxn-113, Rx-601, and Rx-801 were parepared. The Anesthetic activity of ketamine derivatives was determined using tail immersion test and the results were compared with ketamine. For biological activity of the antimicrobial complexes, Agar plate dilution test was performed in order to evaluate the Minimum Inhibitory Concentration (MIC) of ligand and newly synthesized derivatives The antibacterial effect of ketamine was studied using six different strains of bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Entecoccus faecalis, Streptococcus pyogenes, Pseudomonas aeruginosa and Escherichia coli). Ciprofloxacin was used as a control to verify the methodology.
Results:
Comparison of the tail immersion and formalin tests data indicated that derivatives had comparable anesthetic activity to that of Ketamine. The antibacterial effects of all compounds were relatively lower than ciprofloxacin. The derivatives Rxns 102, 113, 103, 601 and 801 demonstrated better effects on bacterial strains of S. aureus and S. epidermidis. However, only Rxn 113 showed better results against E. faecalis.
Conclusions:
Ketamine not only showed profound anesthetic action but also had a strong inhibitory effect on growth of gram positive bacteria Staphylococcus epidermidis