Angiogenesis is the process of formation of new blood vessels from the pre-existing blood vessels controlled by various growth regulators. Disturbances of this regulatory process, by different physiological and pathological disorders results in angiogenesis which leads to the progression of tumor formation. The present study was aimed to explore the effect of Morus nigra (Berry), on angiogenesis by using CAM assay on chicken embryo model. Fertilized eggs were incubated on the 5th day at 37 ºC and dose of different dilutions 0.03%, 0.05%, 0.1%, and 0.5% of this plant extract was applied on 6th day. Evaluation of PBVs, SBVs and TBVs, 3D roughness parameters, angular spectrum and abbot curve of treated CAMs was made on 7th day by image probing system (SPIP). Morus nigra showed antiangiogenic effect by reducing the diameter of CAM blood vessels by applying the dilutions of 0.03%, 0.05%, 0.1%, and 0.5% while significant results were obtained at dilution of 0.5%.